Short-Term Preservation of Mouse Oocytes at 5.DEG.C..
نویسندگان
چکیده
منابع مشابه
Short-term Preservation of Porcine Oocytes in Ambient Temperature: Novel Approaches
The objective of this study was to evaluate the feasibility of preserving porcine oocytes without freezing. To optimize preservation conditions, porcine cumulus-oocyte complexes (COCs) were preserved in TCM-199, porcine follicular fluid (pFF) and FCS at different temperatures (4°C, 20°C, 25°C, 27.5°C, 30°C and 38.5°C) for 1 day, 2 days or 3 days. After preservation, oocyte morphology, germinal ...
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15 صفحه اولImproved hypothermic short-term storage of isolated mouse islets by adding serum to preservation solutions
Preserving isolated islets at low temperature appears attractive because it can keep islet quantity comparable to freshly isolated islets. In this study, we evaluated the effect of serum as an additive to preservation solutions on islet quality after short-term hypothermic storage. Isolated mouse islets were preserved at 4°C in University of Wisconsin solution (UW) alone, UW with serum, M-Kyoto...
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Down syndrome (DS) is a genetic disorder arising from the presence of a third copy of the human chromosome 21 (Hsa21). Recently, O'Doherty and colleagues in an earlier study generated a new genetic mouse model of DS (Tc1) that carries an almost complete Hsa21. Since DS is the most common genetic cause of mental retardation, we have undertaken a detailed analysis of cognitive function and synapt...
متن کاملThe Optimization of Short-Term Hepatocyte Preservation Before Transplantation
BACKGROUND No optimal methods for short-term hepatocyte preservation have been established. We have recently developed a prominent oxygen-permeable bag (Tohoku Device [TD]) for pancreatic islet culture and transplantation. In this study, we investigated whether TD is also effective for hepatocyte preservation and tried to optimize other conditions. METHODS Hepatocytes were preserved in the fo...
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ژورنال
عنوان ژورنال: Experimental Animals
سال: 2001
ISSN: 1341-1357,1881-7122
DOI: 10.1538/expanim.50.441